추천 제품
일반 설명
애플리케이션
- To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.
- Reverse transcription and cDNA amplification
- For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles
- Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)
- qPCR
- microarray analysis
- cloning
특징 및 장점
- Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
- Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
- Contains all needed components for cDNA amplification
- Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
- Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism
원리
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
문서
Transplex Whole Transcriptome Amplification (WTA2) exponentially amplifies RNA producing a double-stranded cDNA library while precisely maintaining differential levels of individual transcripts in test and reference samples.
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
프로토콜
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
관련 콘텐츠
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
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