SRE0093

β-Glucuronidase from limpets (Patella vulgata)

aqueous solution

• 동의어(들): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• 효소 위원회 번호: 3.2.1.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in proprietary host
• Quality Level: 300
• 형태: aqueous solution
• 농도: 100000-200000 units/mL
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

애플리케이션

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.

Poster presentation comparing the hydrolysis efficiency of novel beta-glucuronidase enzymes from recombinant limpet and recombinant E. coli sources, click at the following poster link: Hydrolysis Efficiency Evaluation of Novel Recombinant Limpet and E. coli Beta-Glucuronidase Enzymes

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

특징 및 장점

Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

물리적 형태

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable