QPBCA
QuantiPro™ BCA Assay Kit
for 0.5-30 μg/ml protein
Synonym(s):
BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
About This Item
Recommended Products
Application
Features and Benefits
- Accurate across a broad range of protein concentrations
- High sensitivity; linear response form 0.5 to 30 μg/ml of protein
- Stable color complex
- Reduced susceptibility to detergents
Principle
Storage and Stability
Legal Information
Kit Components Only
- Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL
Kit Components Also Available Separately
- P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS
Application
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
Storage Class Code
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
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Articles
The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined with advances in mass spectrometry, have decreased concerns of sample complexity, and directed more focus towards high-throughput techniques.
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.
Related Content
A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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