HSANGERV

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Human, Virus Format

• UNSPSC Code: 12352200

Properties

• Quality Level: 200
• packaging: pkg of 10 μL (384-well plate)
• concentration: 1x10⁶ VP/ml (via p24 assay)
• application(s): CRISPR
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation

Features and Benefits

• Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
• Simplify the workflow with puromycin selection
• Illuminate CRISPR-expressing cells with BFP

Additional Features

• Better, not bigger: Two optimized clones per gene reduces the time, cost, and scale of screening experiments
• Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
• Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Packaging

384-well plates

Components

Lentivirus Transduction Particles of gRNA-only lenti-based vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones.


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Physical form

10 μl of lentivirus at ≥10⁶ particles/ml in 102×384-well plates

Other Notes

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Recommended products

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Mouse, Virus Format

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable