C9658
Carboxypeptidase G from Pseudomonas sp.
lyophilized powder, ≥3 units/mg protein
Synonym(s):
γ-Glutamyl hydrolase
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About This Item
Recommended Products
form
lyophilized powder
Quality Level
specific activity
≥3 units/mg protein
mol wt
homodimer ~90 kDa
composition
Protein, ~70% biuret
solubility
H2O: soluble 0.5 mg/mL
storage temp.
−20°C
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Application
Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.
Biochem/physiol Actions
Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.
Unit Definition
One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.
Physical form
Contains sodium acetate salt.
Preparation Note
Chromatographically purified
Solutions should be prepared fresh prior to use.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Jeremy P Mallari et al.
Bioorganic & medicinal chemistry, 12(22), 6011-6020 (2004-10-23)
A series of alkyl and aryl phosphonyl, thiophosphonyl, and dithiophosphonyl derivatives of (S)- and (R)-glutamic acid were prepared and examined for inhibitory potency against glutamate carboxypeptidase (carboxypeptidase G). The acquisition of the phosphonamidodithioic acids and the individual phosphonamidothioic acid diastereomers
Takashi Tsukamoto et al.
Bioorganic & medicinal chemistry letters, 12(16), 2189-2192 (2002-07-20)
Phosphonate and phosphinate analogues of N-acylated gamma-glutamylglutamate were tested for the ability to inhibit glutamate carboxypeptidase II (GCP II). All of the compounds inhibit GCP II with IC(50) values in the low nanomolar range. The comparison of the results to
Masaharu Suzuki et al.
Current opinion in plant biology, 11(5), 548-553 (2008-08-12)
Two subfamilies of plant-specific B3 domain transcription factors regulate the fundamental transition between seed and vegetative phases of development. The AFL B3 genes activate the embryo maturation program, while the closely related VAL B3 genes shutdown the AFL network before
Edgar M Pera et al.
Gene expression patterns : GEP, 3(2), 147-152 (2003-04-25)
Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel
The enzymatic hydrolysis of methotrexate and folic acid.
C C Levy et al.
The Journal of biological chemistry, 242(12), 2933-2938 (1967-06-25)
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