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column	Ascentis^® Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U)
column temp.	35 °C
mobile phase	[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile; (15:85, A:B)
flow rate	0.3 mL/min
pressure	1189 psi (82 bar)
sample	300 ng/mL each in acetonitrile
injection	1 µL
detector	MS ESI(+), 100-1000 m/z

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Analysis Note	HILIC mode on Ascentis^® Express OH5 provided rapid separation of neurotransmitters in highly MS-compatible mobile phases. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories	Analytical Chromatography, Clinical, amines, Amines
Featured Industry	Clinical
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability	application for HPLC

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