コンテンツへスキップ
Merck

Matrix metalloproteinase 9 modulates collagen matrices and wound repair.

Development (Cambridge, England) (2015-05-28)
Danny C LeBert, Jayne M Squirrell, Julie Rindy, Elizabeth Broadbridge, Yuming Lui, Anna Zakrzewska, Kevin W Eliceiri, Annemarie H Meijer, Anna Huttenlocher
要旨

Acute and chronic injuries are characterized by leukocyte infiltration into tissues. Although matrix metalloproteinase 9 (Mmp9) has been implicated in both conditions, its role in wound repair remains unclear. We previously reported a zebrafish chronic inflammation mutant caused by an insertion in the hepatocyte growth factor activator inhibitor gene 1 (hai1; also known as spint1) that is characterized by epithelial extrusions and neutrophil infiltration into the fin. Here, we performed a microarray analysis and found increased inflammatory gene expression in the mutant larvae, including a marked increase in mmp9 expression. Depletion of mmp9 partially rescued the chronic inflammation and epithelial phenotypes, in addition to restoring collagen fiber organization, as detected by second-harmonic generation imaging. Additionally, we found that acute wounding induces epithelial cell mmp9 expression and is associated with a thickening of collagen fibers. Interestingly, depletion of mmp9 impaired this collagen fiber reorganization. Moreover, mmp9 depletion impaired tissue regeneration after tail transection, implicating Mmp9 in acute wound repair. Thus, Mmp9 regulates both acute and chronic tissue damage and plays an essential role in collagen reorganization during wound repair.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
モノクロナール抗β-アクチン マウス宿主抗体, clone AC-15, ascites fluid
Sigma-Aldrich
エチレングリコール-ビス(2-アミノエチルエーテル)-N,N,N′,N′-四酢酸, for molecular biology, ≥97.0%
Sigma-Aldrich
3-アミノ安息香酸エチル メタンスルホン酸塩, 98%
Sigma-Aldrich
硫酸マグネシウム, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
エチレングリコール-ビス(2-アミノエチルエーテル)-N,N,N′,N′-四酢酸, ≥97.0%
Sigma-Aldrich
硫酸マグネシウム 溶液, for molecular biology, 1.00 M±0.04 M
Sigma-Aldrich
N-フェニルチオ尿素, ≥98%
Sigma-Aldrich
水酸化カリウム, anhydrous, ≥99.95% trace metals basis
Sigma-Aldrich
硫酸マグネシウム 溶液, BioUltra, for molecular biology
Sigma-Aldrich
過酸化水素 溶液, JIS special grade, 30.0-35.5%
Sigma-Aldrich
過酸化水素 溶液, SAJ first grade, ≥30.0%
Sigma-Aldrich
硫酸マグネシウム, ≥99.99% trace metals basis
Sigma-Aldrich
エチレングリコール-ビス(2-アミノエチルエーテル)-N,N,N′,N′-四酢酸, BioXtra, ≥97 .0%
Sigma-Aldrich
水酸化カリウム 溶液, 0.5 M in ethanol
Sigma-Aldrich
水酸化カリウム 溶液, 1 M
Sigma-Aldrich
水酸化カリウム 溶液, 0.1 M
Sigma-Aldrich
3-アミノ安息香酸エチル, 97%
Sigma-Aldrich
水酸化カリウム 溶液, 5 M
Sigma-Aldrich
水酸化カリウム, JIS special grade, ≥85.0%
Sigma-Aldrich
水酸化カリウム 溶液, 0.1 M in ethanol
Sigma-Aldrich
硫酸マグネシウム, SAJ first grade (dried), ≥99.0%
Sigma-Aldrich
硫酸マグネシウム, SAJ first grade (anhydrous), ≥98.0%
Sigma-Aldrich
水酸化カリウム 溶液, 0.1 M in isopropanol
Sigma-Aldrich
水酸化カリウム 溶液, 0.5 M
Sigma-Aldrich
水酸化カリウム 溶液, 0.02 M in ethanol