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Merck
  • Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes.

Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes.

Biotechnology and bioengineering (1990-07-01)
M Hasegawa, H Kitano, R Nishida, T Kobashi
要旨

Hydrophilic and macroporous polymer resins composed of glycerylmethacrylate, styrene, and divinylbenzene were quite easily modified with isothiocyanate groups using a Friedel-Crafts reaction with 3-chloropropionyl chloride and subsequent nucleophilic reaction with KSCN. Alkaline phosphatase and trypsin could be covalently bound to the isothiocyanate-carrying polymer resins, and the immobilized enzymes obtained were sufficiently active and stable due to a covalent bonding via a spacer group between the carrier resins and the enzymes and also due to a hydrophilic environment around the enzymes. A heterogeneity of the immobilized enzyme was taken into account to interpret the thermal denaturation process of the enzyme immobilized onto the resins.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
3-クロロプロピオニルクロリド, technical grade