コンテンツへスキップ
Merck
  • Simultaneous quantification of reduced and oxidized glutathione in plasma using a two-dimensional chromatographic system with parallel porous graphitized carbon columns coupled with fluorescence and coulometric electrochemical detection.

Simultaneous quantification of reduced and oxidized glutathione in plasma using a two-dimensional chromatographic system with parallel porous graphitized carbon columns coupled with fluorescence and coulometric electrochemical detection.

Journal of chromatography. A (2005-12-27)
Amrit K Sakhi, Kjell Magne Russnes, Sigbjørn Smeland, Rune Blomhoff, Thomas E Gundersen
要旨

A method for the simultaneous quantification of reduced and oxidized glutathione in human plasma employing a two-dimensional chromatographic system with parallel porous graphitized carbon (PGC) columns coupled with fluorescence (FLD) and coulometric electrochemical detection (ED) has been developed. Post-sampling oxidation of reduced glutathione (GSH) was prevented by derivatizing the -SH group with monobromobimane (MBB) and the glutathione-bimane adduct (GSMB) was detected by FLD. Oxidized glutathione (GSSG) was detected by ED optimized to give lowest possible limits of detection (LOD). The method is fully validated and is currently used for determination of GSH, GSSG and its redox potential in different clinical studies.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
L-グルタチオン, 酸化型, ≥98% (HPLC)
Sigma-Aldrich
L-グルタチオン, 酸化型, lyophilized powder
Sigma-Aldrich
L-グルタチオン, 酸化型, BioXtra, ≥98%