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Merck
  • Single nucleotide analysis of cytosine methylation by whole-genome shotgun bisulfite sequencing.

Single nucleotide analysis of cytosine methylation by whole-genome shotgun bisulfite sequencing.

Current protocols in molecular biology (2012-08-09)
Michelle D Johnson, Michael Mueller, Laurence Game, Timothy J Aitman
要旨

Unlike other methods to assess methylation, whole-genome shotgun bisulfite sequencing is used to generate quantitative genome-wide methylation profiles at single-nucleotide resolution. As described in this unit, this method allows for the quantitative measurement of methylation at each cytosine base by treatment of genomic DNA with sodium bisulfite followed by sequencing and alignment of the reads to a reference genome.

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製品内容

Sigma-Aldrich
重亜硫酸ナトリウム, ACS reagent
Sigma-Aldrich
重亜硫酸ナトリウム 溶液, purum, ~40%
Sigma-Aldrich
重亜硫酸ナトリウム, anhydrous, free-flowing, Redi-Dri, ACS reagent
Sigma-Aldrich
重亜硫酸ナトリウム, JIS special grade, 64.0-67.4% SO2 basis
Sigma-Aldrich
重亜硫酸ナトリウム, SAJ first grade, ≥58.5% SO2 basis