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Merck
  • Combination of two hydrophilic interaction chromatography methods that facilitates identification of 2-aminobenzamide-labeled oligosaccharides.

Combination of two hydrophilic interaction chromatography methods that facilitates identification of 2-aminobenzamide-labeled oligosaccharides.

Journal of chromatographic science (2008-01-26)
Göran Karlsson, Elin Swerup, Helena Sandberg
要旨

Hydrophilic interaction chromatography (HILIC) is used to separate 2-aminobenzamide- (2-AB) labeled N-linked oligosaccharides. The glycans of the model protein, bovine fetuin, are identified following comparison of elution patterns of seven 2-AB-labeled glycan standards, of which two are of the high-mannose type and five are of the complex type. The combination of two HILIC methods, using an Amide-80 column, having different resolutions and selectivities, markedly facilitates the identification of the fetuin glycans. These HILIC methods are suitable for obtaining glycan profiles of complex mixtures.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
A3グリカン, from bovine, ≥90% (HPLC)