コンテンツへスキップ
Merck

Progressive axonopathy when oligodendrocytes lack the myelin protein CMTM5.

eLife (2022-03-12)
Tobias J Buscham, Maria A Eichel-Vogel, Anna M Steyer, Olaf Jahn, Nicola Strenzke, Rakshit Dardawal, Tor R Memhave, Sophie B Siems, Christina Müller, Martin Meschkat, Ting Sun, Torben Ruhwedel, Wiebke Möbius, Eva-Maria Krämer-Albers, Susann Boretius, Klaus-Armin Nave, Hauke B Werner
要旨

Oligodendrocytes facilitate rapid impulse propagation along the axons they myelinate and support their long-term integrity. However, the functional relevance of many myelin proteins has remained unknown. Here, we find that expression of the tetraspan-transmembrane protein CMTM5 (chemokine-like factor-like MARVEL-transmembrane domain containing protein 5) is highly enriched in oligodendrocytes and central nervous system (CNS) myelin. Genetic disruption of the Cmtm5 gene in oligodendrocytes of mice does not impair the development or ultrastructure of CNS myelin. However, oligodendroglial Cmtm5 deficiency causes an early-onset progressive axonopathy, which we also observe in global and tamoxifen-induced oligodendroglial Cmtm5 mutants. Presence of the WldS mutation ameliorates the axonopathy, implying a Wallerian degeneration-like pathomechanism. These results indicate that CMTM5 is involved in the function of oligodendrocytes to maintain axonal integrity rather than myelin biogenesis.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
抗APP A4抗体、APP {NT}のa.a. 66-81、クローン22C11, clone 22C11, Chemicon®, from mouse
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
抗アミロイド抗体、β 1-40/42, serum, Chemicon®
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
抗CNP抗体 マウス宿主抗体, purified immunoglobulin, buffered aqueous solution
カタログ番号
容量
在庫状況
単価
数量