Capillary zone electrophoresis of oligosaccharides derivatized with N-(4-aminobenzoyl)-L-glutamic acid for ultraviolet absorbance detection.

A charged and strongly UV-absorbing tag, N-(4-aminobenzoyl)-L-glutamic acid) (ABG), was coupled to oligosaccharides by reductive amination under mild conditions. The effectiveness of ABG as a derivatization agent is shown through the separation of isomaltooligosaccharides from a dextran hydrolysate. The minimum detectable quantities in the subpicomole range are demonstrated.