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Merck

Immunofluorescence stimulated emission depletion microscopy.

Nature biotechnology (2003-10-21)
Marcus Dyba, Stefan Jakobs, Stefan W Hell
要旨

We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.