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Merck
  • Over-expression of Flt3 induces NF-kappaB pathway and increases the expression of IL-6.

Over-expression of Flt3 induces NF-kappaB pathway and increases the expression of IL-6.

Leukemia research (2005-06-28)
Shinichiro Takahashi, Hideo Harigae, Keiko Kumura Ishii, Mitsue Inomata, Tohru Fujiwara, Hisayuki Yokoyama, Kenichi Ishizawa, Junichi Kameoka, Jonathan D Licht, Takeshi Sasaki, Mitsuo Kaku
要旨

Activating mutations or over-expression of the Flt3 is prevalent in acute myeloblastic leukemia (AML), associated with activation of Ras/MAP kinase and other signaling pathways. In this study, we addressed the role of Flt3 in the activation of nuclear factor-kappa B (NF-kappaB), which is a target molecule of these kinase pathways. In BaF3 cells stably expressing Flt3, a NF-kappaB-responsive reporter was upregulated and its target gene, IL-6, was increased by the involvement of Flt3-ERK/MAPK-NF-kappaB pathway. Furthermore, we found a modest positive correlation (r=0.35, p=0.096) between Flt3 and IL-6 mRNA expression in 24 AML specimens. These results suggest a role of Flt3 over-expression in NF-kappaB pathway.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
Flt3 active human, recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)