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  • A fast signal-induced activation of Poly(ADP-ribose) polymerase: a novel downstream target of phospholipase c.

A fast signal-induced activation of Poly(ADP-ribose) polymerase: a novel downstream target of phospholipase c.

The Journal of cell biology (2000-07-26)
S Homburg, L Visochek, N Moran, F Dantzer, E Priel, E Asculai, D Schwartz, V Rotter, N Dekel, M Cohen-Armon
要旨

We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein found only in eukaryotes, exclusively catalyzes polyADP-ribosylation of DNA-binding proteins, thereby modulating their activity. Activation of PARP, reportedly induced by formation of DNA breaks, is involved in DNA transcription, replication, and repair. Our findings demonstrate an alternative mechanism: a fast activation of PARP, evoked by inositol 1,4,5,-trisphosphate-Ca(2+) mobilization, that does not involve DNA breaks. These findings identify PARP as a novel downstream target of phospholipase C, and unveil a novel fast signal-induced modification of DNA-binding proteins by polyADP-ribosylation.

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デオキシリボヌクレアーゼI RNアーゼ不含 溶液 ウシ膵臓由来