GE17-5199-01

MabSelect^™

Cytiva 17-5199-01, pack of 25 mL

• 別名: MabSelect Column
• UNSPSCコード: 23151817
• NACRES: NA.56

特性

• ligand: recombinant protein A (E. coli)
• 包装: pack of 25 mL
• メーカー／製品名: Cytiva 17-5199-01
• Matrix: highly cross-linked agarose
• 平均部品サイズ: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning in place: 2-12
• 動作範囲: 3-10
• キャパシティ: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 適合性: suitable for bioprocess medium
• 保管温度: 2-8°C

詳細

詳細

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

MabSelect^™ is a protein A affinity medium designed for capturing MAbs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

アプリケーション

MabSelect^™ is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect^™ is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect^™ has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.

The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect^™ is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.

The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect^™ an excellent choice for purification of MAbs at process scale.

MabSelect^™ is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect^™ meets industrial demands with security of supply and comprehensive technical and regulatory support.

特徴および利点

• High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
• Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
• Low non-specific binding resulting in Low levels of impurities in the product eluate pool
• BioProcess medium supported for industrial applications and well-established in approved processes.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

MabSelect is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids