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テクニック
protein quantification: suitable
single molecule counting: suitable
適合性
suitable for immunoassay
検出方法
fluorometric
詳細
Single Molecule Counting (TM=["SMC"]) Technology: Reduced background + increased signal
TM=["SMC"] technology provides maximum immunoassay performance while following a workflow similar to traditional ELISA technology. By combining a unique assay elution step and robust digital counting, TM=["SMC"] technology achieves improved signal to noise ratios over traditional immunoassay technologies. The TM=["SMC"] technology thus provides enhanced quantification at both low and high levels of expression on one complete system.
TM=["SMC"] Assay Workflow
During the capture and detection steps, specific antibodies translate each biomarker into a signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The TM=["SMCxPRO"] system is the second generation instrument supporting TM=["SMC"] technology assays. The TM=["SMCxPRO"] technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).
Digital counting improves sensitivity and dynamic range
The TM=["SMCxPRO"] instrument captures the sum of all digital events counted above a background threshold. The TM=["SMCxPRO"] instrument digital event algorithm computes digital events across a spectrum of time series for a single standard curve, thus improving assay sensitivity
and extending the dynamic measuring range beyond what can be achieved with traditional technologies.
TM=["SMC"] technology provides maximum immunoassay performance while following a workflow similar to traditional ELISA technology. By combining a unique assay elution step and robust digital counting, TM=["SMC"] technology achieves improved signal to noise ratios over traditional immunoassay technologies. The TM=["SMC"] technology thus provides enhanced quantification at both low and high levels of expression on one complete system.
TM=["SMC"] Assay Workflow
During the capture and detection steps, specific antibodies translate each biomarker into a signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The TM=["SMCxPRO"] system is the second generation instrument supporting TM=["SMC"] technology assays. The TM=["SMCxPRO"] technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).
Digital counting improves sensitivity and dynamic range
The TM=["SMCxPRO"] instrument captures the sum of all digital events counted above a background threshold. The TM=["SMCxPRO"] instrument digital event algorithm computes digital events across a spectrum of time series for a single standard curve, thus improving assay sensitivity
and extending the dynamic measuring range beyond what can be achieved with traditional technologies.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
試験成績書（COA）
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