ZRB2294

Anti-Catenin α-1 Antibody, clone 1D15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Alpha E-catenin, Cadherin-associated protein, Catenin alpha-1, Renal carcinoma antigen NY-REN-13
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D15, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): feline, monkey, mouse, bovine, rat, porcine, canine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001277236.1
• UniProt accession no.: P35221
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CTNNA1(1495)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Catenin -1. It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Catenin -1.

Application

Quality Control Testing

Evaluated by Western Blotting in Human Small Intestine tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Catenin -1 in Human Small Intestine tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Catenin -1 in Human small intestine tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Catenin -1 peptide with a KD of 6.5 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Catenin alpha-1 (UniProt: P35221; also known as Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-Ren^™-13) is encoded by the CTNNA1 gene (Gene ID: 1495) in human. Catenin α-1 is a ubiquitously expressed protein that can exist as a monomer of a homodimer. The monomeric form preferentially binds to catenin β1 and the homodimeric form binds to actin. Catenin α-1 associates with the cytoplasmic domain of a variety of cadherins, and this association produces a complex that is linked to the actin filament network and appears to be of importance for cadherins cell-adhesion properties. At cell junctions, it is essential for cell cohesion and tissue organization. It also plays a role in contact-dependent growth inhibition and can antagonize Wnt/β-catenin-mediated transcription. Usually, cells contain a significant amount of cadherin-free α-catenin, and this pool of free α-catenin can impact actin dynamics, such as limiting Arp2/3-based actin polymerization and severing by cofilin that ultimately promotes the assembly and stabilization of unbranched filament bundles. Loss of function of CTNNA1 gene has been reported in several cases of breast cancer and some cases of gastric cancers. Three isoforms of Catenin α-1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Clark, DF., et al. (2020). Genet Med 22(5); 840-846; Daugherty, RL., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(14); 5260-5265).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

Ren is a trademark of Huntsman Advanced Materials GmbH

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable