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Safety Information

ABF32

Sigma-Aldrich

Anti-Fibroblast-specific Protein 1 (S100A4) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human, horse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), bovine (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_002952

UniProt accession no.

P26447

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... S100A4(6275)

General description

S100A4, also known as Fibroblast-specific Protein 1, is a member of the Ca2+- dependent S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 is a metastasis factor that may function in motility, invasion, and tubulin polymerization. Increased expression of S100A4 has been associated with various types of cancer.

Specificity

This antibody recognizes S100A4 at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human S100A4.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected S100A4 in mouse neural stem cells.
Research Category
Stem Cell Research

Inflammation & Immunology
Research Sub Category
Ion & Transport Channels

Inflammation & Autoimmune Mechanisms
This Anti-Fibroblast-specific Protein 1 (S100A4) Antibody is validated for use in WB, IC for the detection of Fibroblast-specific Protein 1 (S100A4).

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.

Target description

~12 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABF32:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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