ZRB1446

Anti-GNA-11 (G alpha 11) Antibody, clone 1F16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): G alpha-11, G-protein subunit alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha, Guanine nucleotide-binding protein subunit alpha-11
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F16, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 42.12 kDa; observed mol wt 40 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P35243
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GNA11(2767)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1F16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Guanine nucleotide-binding protein subunit alpha-11 (Ga 11). It targets an epitope within 10 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region of human Guanine nucleotide-binding protein subunit alpha-11 (Ga 11).

Application

Anti-GNA-11 (G 11), clone 1F16 ZooMAb, Cat. No. ZRB1446, is a recombinant Rabbit monoclonal antibody that specifically targets GNA-11 (G protein alpha 11) and is tested in Immunocytochemistry and Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GNA-11 (Ga 11) in HeLa, NIH3T3, and rat brain microsomal lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GNA-11 (Ga 11) in human placenta tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GNA-11 (Ga 11) in HeLa, A431, HUVEC and NIH 3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Guanine nucleotide-binding protein subunit alpha-11(UniProt: P29992; also known as G alpha-11, G-protein subunit alpha-11, Guaninenucleotide-binding protein G(y) subunit alpha) is encoded by the GNA11 (alsoknown as GA11) gene (Gene ID: 2767) in human. G proteins are composed of 3units; alpha (36-52 kDa), beta (35-36 kDa), and gamma (8-10 kDa). The alphachain contains the guanine nucleotide binding site. When bound to GDP, G alpha associates withthe beta-gamma dimer to form the inactive heterotrimer. Ligand/agonist bindingto a GPCR alters the receptor’s conformation allowing it to catalyze theexchange of GDP for GTP on the alpha subunit of the G protein coupled to thereceptor. The binding of GTP produces aconformational change in the G protein alpha subunit resulting in thedissociation of the receptor-G protein complex and freeing the alpha andbeta-gamma subunits to interact with their target effectors. The slow intrinsic GTPase activity of thesubunit hydrolyzes the bound GTP to GDP ending the interaction of the subunitwith its’ effector. The GDP bound alphasubunit interacts strongly with beta-gamma subunits and thus GTP hydrolysispresumably also terminates beta-gamma mediated signaling by promoting theassociation of the GDP-bound subunits with beta-gamma dimers. The G alpha 11 targets phospholipase C (PLC)that cleaves phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] to generateinositol trisphosphate (IP3) and membrane bound diacylglycerol (DAG). ThisZooMAb^® recombinant monoclonal antibody, generated by our propriety technology,offers significantly enhanced specificity, affinity, reproducibility, andstability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable