T9650

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

• Synonym(s): TAE buffer
• MDL number: MFCD00236357
• UNSPSC Code: 41105319
• PubChem Substance ID: 329826908
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile; 0.2 μm filtered
• product line: BioReagent
• form: solution
• suitability: suitable for gel electrophoresis (after dilution to working concentration)
• foreign activity: Protease, none detected; RNAse, none detected
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• InChI key: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Description

Application

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Other Notes

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H373
• Precautionary Statements: P260 - P313 - P501
• Hazard Classifications: STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable