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Safety Information

SRP5127

Sigma-Aldrich

RhoA, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

ARH12, ARHA, RHO12, RHOH12

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~23 kDa

NCBI accession no.

NM_001664

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... RHOA(387)

General description

Ras homolog family member A (RhoA) is a small GTPase which is part of the Rho gene family. The gene encoding it is localized on human chromosome 3p21.31.

Application

RhoA, His tagged human has been used for in vitro kinase assay.

Biochem/physiol Actions

Ras homolog family member A (RhoA) interacts with protein kinases and serves as a target of activated GTPase. RhoA plays an important role in the initial events of cell protrusion. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton. The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signaling using the viral protein F11 and this can enhance the spread of infection. The protein has a role in basal body docking and planar polarization.

Physical form

Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

SRP5127-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells.
Sedzinski J
Journal of Cell Science (2017)
Connective Tissue Growth Factor in Regulation of RhoA Mediated Cytoskeletal Tension Associated Osteogenesis of Mouse Adipose-Derived Stromal Cells
Yue Xu
PLoS ONE (2010)
Activation of RHOA gene transcription in epithelial tumors may be caused by gene amplification and/or demethylation of its promotor region
Molecular Biology (2006)
Coordination of Rho GTPase activities during cell protrusion.
Machacek M
Nature (2009)
Phosphodiesterase 5 Inhibition Limits Doxorubicin-induced Heart Failure by Attenuating Protein Kinase G Ia Oxidation*
Oleksandra P
The Journal of Biological Chemistry (2016)
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