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L4146

Sigma-Aldrich

Leptin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

OB

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About This Item

CAS Number:
177404-21-6
MDL number:
MFCD01094205
UNSPSC Code:
51111800
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

100

sterility

sterile; 0.2 μm filtered

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤3.0 ng/mL ED50/EC50

mol wt

predicted mol wt ~16 kDa

impurities

≤1.0 EU/μg endotoxin

UniProt accession no.

P41159

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... LEP(3952)

Related Categories

General description

The gene LEP (leptin) is mapped to human chromosome 7q31. It is a 16kDa peptide. Leptin is released in the circulation and goes to the CNS (central nervous system). In CNS, it works by associating with leptin receptor.

Application

Leptin human has been used:
  • to study the effect of leptin on ACAT1 (acyl-coenzyme A:cholesterol acyltransferase 1) expression and cholesterol efflux from primary cultured human macrophages
  • to study the effect of leptin on expression of IGFBP2 (insulin growth factor binding protein 2) in skeletal muscle
  • to study the effect of leptin on HLA-G (human leukocyte antigen G) expression in placental trophoblasts
  • to study the effect of leptin on in vitro maturation, fertilization and development of horse oocytes after ICSI (intracytoplasmic sperm injection)

Biochem/physiol Actions

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Leptin is believed to play a role in normal pregnancy development.
Mutations in LEP (leptin) are linked with type-2 diabetes. Presence of high levels of leptin in plasma is related to adiposity.

Physical form

Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L4146-1MG-PW:
L4146-1MG:
L4146-VAR:
L4146-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reza Meshkani et al.
Clinical laboratory, 62(1-2), 89-96 (2016-03-26)
The leptin (LEP G2548A) and leptin receptor (LEPR Q223R) gene polymorphisms have been variably associated with type 2 diabetes (T2D) in different populations. In this study we hypothesized that these variants might be associated with T2D and related metabolic traits
Eiji Kashiwagi et al.
American journal of translational research, 12(6), 3033-3045 (2020-07-14)
Adipocytokines such as leptin and adiponectin have functions in metabolism as well as the development and progression of various types of malignancies. However, little is known about their role in bladder cancer. In this study, we investigated whether leptin, adiponectin
Leptin action in normal and pathological pregnancies
Peerez PA, et al.
Journal of Cellular and Molecular Medicine, 22(2), 716-716 (2018)
Elisabetta Trevellin et al.
Oncotarget, 6(13), 11203-11215 (2015-04-11)
Obesity is associated with cancer risk in esophageal adenocarcinoma (EAC). Adipose tissue directly stimulates tumor progression independently from body mass index (BMI), but the mechanisms are not fully understood. We studied the morphological, histological and molecular characteristics of peritumoral and
Masafumi Kurajoh et al.
Cardiovascular diabetology, 14, 117-117 (2015-09-05)
It has been shown that visceral fat accumulation is associated with autonomic dysfunction, though the precise mechanism remains unclear. A recent basic study found that leptin can directly modulate autonomic function through the dorsomedial hypothalamus in relation to obesity. Here
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