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Safety Information

SRP0364

Sigma-Aldrich

mTOR/Raptor/MLST8 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥50% (SDS-PAGE)

form

aqueous solution

mol wt

137 kDa (mTOR)
 150 kDa (Raptor)
 37 kDa (MLST8)

packaging

pkg of 10 μg

manufacturer/tradename

Sigma-Aldrich

technique(s)

cell based assay: suitable

solubility

water: soluble

NCBI accession no.

NM_004958 (mTOR)
NM_020761 (Raptor)
NM_022372 (MLST8)

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MLST8(64223) , MTOR(2475) , RPTOR(57521)

General description

This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.

Application

Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.

Biochem/physiol Actions

Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

SRP0364-10UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunoregulatory functions of mTOR inhibition.
Thomson AW
Nature Reviews: Immunology, 9(5), 324-337 (2009)
Belen Rubio-Viqueira et al.
Advances in experimental medicine and biology, 587, 309-327 (2006-12-14)
The mammalian target of rapamycin (mTOR) is involved in the control of cellular growth and proliferation. Abnormal activation of signaling pathways both proximal and distal to this kinase occurs frequently in human cancer suggesting that mTOR is an attractive target
mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E.
Fingar DC
Molecular and Cellular Biology, 24(1), 200-216 (2004)
mLST8 Promotes mTOR-Mediated Tumor Progression.
Kakumoto K
PLoS ONE, 10(4), e0119015-e0119015 (2015)
Lourdes Toral-Barza et al.
Biochemical and biophysical research communications, 332(1), 304-310 (2005-05-18)
The mammalian target of rapamycin (mTOR/TOR) is implicated in cancer and other human disorders and thus an important target for therapeutic intervention. To study human TOR in vitro, we have produced in large scale both the full-length TOR (289 kDa)
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