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Key Documents

Safety Information

SRP0294

Sigma-Aldrich

FAP Active human

recombinant, expressed in baculovirus, ≥50% (SDS-PAGE)

Synonym(s):

FAPA, Fibroblast activation protein, α, Melanoma membrane-bound gelatinase 170 kDa, Seprase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in baculovirus

Assay

≥50% (SDS-PAGE)

form

aqueous solution

specific activity

≥10.2 pmol/min-g

mol wt

85.5 kDa

NCBI accession no.

NM_004460

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... FAP(2191)

General description

Human FAP (GenBank Accession No. NM_004460), (amino acids 29-end) with C-terminal HIS tag, MW = 85.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Active human FAP is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human FAP has been used in a study to assess the role of FAP48 in HIV-associated lipodystrophy. Active human FAP has also been used in a study to investigate the current state of domino transplantation in surgical procedures and de novo amyloid neuropathy.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200mM Imidazole, 3 mM DTT and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

Cartagena Act

Cartagena Act Listed

JAN Code

SRP0294-25UG:
SRP0294-10UG:

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Certificates of Analysis (COA)

Lot/Batch Number
8010040911
80100002001
8010002001

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T Lisman et al.
Journal of thrombosis and haemostasis : JTH, 10(8), 1624-1630 (2012-05-31)
The platelet count varies considerably between individuals, but within an individual the platelet count is remarkably stable over time. Mechanisms controlling the platelet count are not yet established. In the present study, we tested the hypothesis that the liver is
Sarah E Herlihy et al.
Protein science : a publication of the Protein Society, 26(3), 578-585 (2016-12-29)
Autocrine proliferation repressor protein A (AprA) is a protein secreted by Dictyostelium discoideum cells. Although there is very little sequence similarity between AprA and any human protein, AprA has a predicted structural similarity to the human protein dipeptidyl peptidase IV
Current state of domino transplantation in Japan in terms of surgical procedures and de novo amyloid neuropathy
Asonuma, K., et al.
Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis, 1, 75-77 (2012)
Peio Errarte et al.
PloS one, 11(12), e0169105-e0169105 (2016-12-30)
Clear cell renal cell carcinoma (CCRCC) is a heterogeneous and complex disease that frequently develops distant metastases. Fibroblast activation protein (FAP) is a serine peptidase the expression of which in cancer-associated fibroblasts has been associated with higher risk of metastases
Vincenzo Esposito et al.
Journal of cellular biochemistry, 113(11), 3446-3454 (2012-06-09)
The highly active antiretroviral therapy (HAART) can cause a metabolic syndrome consisting of lipodystropy/lipoatrophy, dyslipidemia, and type 2 diabetes mellitus with an increased cardiovascular risk. The pathogenetic bases of HAART-associated lipodystrophy are poorly known. A genetic screen was used to
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