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P1867

Sigma-Aldrich

Plasmin from human plasma

lyophilized powder, ≥2.0 units/mg protein

Synonym(s):

Fibrinolysin

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About This Item

CAS Number:
9001-90-5
Enzyme Commission number:
3.4.21.7 (BRENDA, IUBMB)
EC Number:
232-640-3
MDL number:
MFCD00131921
UNSPSC Code:
12352204
eCl@ss:
42030239
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥2.0 units/mg protein

impurities

HIV, Hepatitis B and Hepatitis C, tested negative

UniProt accession no.

P00747

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

human ... PLG(5340)

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General description

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes.

Application

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin.

Biochem/physiol Actions

It is inhibited by α 2-antiplasmin and its interaction with fibrin is blocked. It digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells.
Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.
pH Optimum: 8.5
pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity
Temperature Optimum: 37 °C with rapid inactivation at 56 °C

Packaging

Package size based on protein content

Unit Definition

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing sodium phosphate and Trehalose.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P1867-VAR:
P1867-500UG-PW:
P1867-150UG-PW:
P1867-BULK:
P1867-750UG:
P1867-150UG:
P1867-500UG:
P1867-1500UG-PW:
P1867-750UG-PW:
P1867-1500UG:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM1141
SLCL6603
SLCL2286
SLCJ1058
SLCJ0878

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α2-Antiplasmin from human plasma lyophilized powder, ≥5 units/mg protein

Sigma-Aldrich

A8849

α2-Antiplasmin from human plasma

Silke Haerteis et al.
The Journal of general physiology, 140(4), 375-389 (2012-09-12)
Proteolytic activation of the epithelial sodium channel (ENaC) involves cleavage of its γ subunit in a critical region targeted by several proteases. Our aim was to identify cleavage sites in this region that are functionally important for activation of human
Tatiana Syrovets et al.
Journal of leukocyte biology, 92(3), 509-519 (2012-05-09)
The serine protease plasmin generated from its zymogen plasminogen is best known for its function as a key enzyme of the fibrinolytic cascade. However, beyond fibrinolysis, plasmin has a number of crucial functions in a variety of processes, including inflammation.
S Müllertz et al.
The Biochemical journal, 159(3), 545-553 (1976-12-01)
A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced.
On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin.
B Wiman et al.
The Journal of biological chemistry, 254(18), 9291-9297 (1979-09-25)
Shu He et al.
British journal of haematology, 160(6), 806-816 (2013-01-31)
To assess whether Haemocomplettan(®) (fibrinogen concentrate) or Fibrogammin(®) (Factor XIII concentrate) can be used to manage bleeding complications of antithrombotic treatment, we examined a normal plasma pool spiked with AR-H067637 (thrombin inhibitor) or rivaroxaban (activated factor X-inhibitor), to which one
View All Related Papers

Protocols

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

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