Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

SML1633

Sigma-Aldrich

7-AAD

≥97% (HPLC), liquid (solution in DMSO:H2O (1:1)), fluorescent indicator

Synonym(s):

7-Aminoactinomycin D Ready Made Solution, 7-Aminodactinomycin Ready Made Solution

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C62H87N13O16
CAS Number:
Molecular Weight:
1270.43
UNSPSC Code:
12352200
NACRES:
NA.77

product name

7-AAD Ready Made Solution, 1 mg/mL

form

liquid (solution in DMSO:H2O (1:1))

Quality Level

storage condition

protect from light

concentration

1 mg/mL

storage temp.

−20°C

InChI

1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)

InChI key

YXHLJMWYDTXDHS-UHFFFAOYSA-N

General description

Free form:
Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm
Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm
DNA Complex:
Excitation: 543 nm; 555 nm
Emission: 655 nm; 665 nm

Application

Fluorescent DNA stain. A featured product for Apoptosis research.

Biochem/physiol Actions

7-AAD is used in flow cytometry analysis of viable cells. 7-ADD is a fluorescent indicator that undergoes a spectral shift upon association with DNA. Su, X., et al. used FITC and PE-conjugated antibodies to stain cell surface markers. After cell-surface marker staining, cells were further stained with 10 μg/ml of 7-AAD in PBS on ice for 30 minutes. After washing with PBS twice, the cells were fixed in 1% paraformaldehyde supplemented with 50 μg/ml actinomycin D. Non-apoptotic cells were 7-AAD negative. 7-AAD like its parent molecule, Actinomycin D, is a DNA-intercalator with growth-inhibitory properties.
7-amino-actinomycin D (7-AAD) is a DNA dye. It can be used as a replacement for propidium iodide (PI) for the discrimination of dead cells. This dye is ideal for dead cell discrimination in long-lasting experiments, as it is efficiently exempted by intact cells and has a high DNA binding constant.

Reconstitution

Solution of 1 mg/ml in DMSO:H2O (1:1) is stable for at least 2 years at −20 °C without loss of activity. According to Su et al., the provided solution should be diluted to 10 μg/ml (1:100) working concentration in PBS and is therefore sufficient for 100 stain reactions.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SML1633-1ML:
SML1633-VAR:
SML1633-BULK:
SML1633-1ML-PW:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Dead cell discrimination with 7?amino?actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry.
Schmid I, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 13(2), 204-208 (1992)
Jinwoo Seong et al.
Development (Cambridge, England), 145(14) (2018-06-29)
Mammary glands develop through primary ductal elongation and side branching to maximize the spatial area. Although primary ducts are generated by bifurcation of terminal end buds, the mechanism through which side branching occurs is still largely unclear. Here, we show

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service