SAB4200101

Anti-AS160 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Akt substrate of 160 kDa, Anti-TBC1D4 (TBC1 domain family, member 4)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): indirect immunofluorescence: 8-16 μg/mL using HEK-293T cells; western blot: 0.8-1.6 μg/mL using extracts of HEK-293T cells over expressing human AS160
• UniProt accession no.: O60343
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TBC1D4(9882) ; mouse ... Tbc1d4(210789)

Description

General description

AS160 (Akt substrate of 160 kDa, also known as TBC1 domain family member 4 (TBC1D4)) gene is mapped to human chromosome 13q22.2 and encodes a Rab guanosine triphosphatase (GTPase)-activating proteins (RabGAP) protein. AS160 is expressed in many differentiated tissues such as adipocytes and skeletal muscles.

Application

Anti-AS160 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

AS160 is known to play an inhibitory role in the transport of glucose transporter 4 (GLUT4) vesicles. It is associated with GLUT4 vesicles in basal states and dissociates in response to insulin. Reduced expression of AS160 in adipocytes has been shown to increase plasma membrane levels of GLUT4 in an insulin-dependent manner.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable