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R8779

Sigma-Aldrich

Anti-Rab7 antibody, Mouse monoclonal

~2 mg/mL, clone Rab7-117, purified from hybridoma cell culture

Synonym(s):

Rab7 Antibody, Rab7 Antibody - Monoclonal Anti-Rab7 antibody produced in mouse, Anti-Ras-associated protein

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Rab7-117, monoclonal

form

buffered aqueous solution

mol wt

~24 kDa

species reactivity

bovine, human, monkey, mouse, chicken, rat

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa total cell extract

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB7A(7879)
mouse ... Rab7(19349)
rat ... Rab7a(29448)

General description

Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). This family belongs to the rat sarcoma (Ras) superfamily of small GTPases. Rab proteins contain conserved regions involved in guanine nucleotide binding and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. The gene Rab7a in human chromosome is mapped to 3q21.3.

Specificity

Monoclonal Anti-Rab7 recognizes human, monkey, bovine, rat, mouse, and chicken Rab7.

Application

Monoclonal Anti-Rab7 antibody produced in mouse may be used in ELISA, immunoblotting (~24 kDa), immunoprecipitation, immunohistochemistry, immunofluorescence and immunocytochemistry.

Biochem/physiol Actions

Rab GTPases are central regulators of membrane trafficking between different subcellular compartments in eukaryotic cells. The regulatory capacity of Rab GTPases depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Activation of Rab proteins is coupled to their association with intracellular membranes, allowing them to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin and tubulin-dependent vesicle movement, and membrane fusion. Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are Rab-interacting lysosome protein (RILP) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rab-7-interacting ring finger protein (Rabring7) and the hVPS34/p150 complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R8779-200UL:
R8779-VAR:
R8779-BULK:
R8779-100UL:
R8779-25UL:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Rab GTPase family
Stenmark H and Olkkonen VM
Genome Biology, 2(5), reviews3007-reviews3007 (2001)
CMT2B-associated Rab7 mutants inhibit neurite outgrowth
Cogli L, et al.
Acta Neuropathologica, 120(4), 491-501 (2010)
Rab7b, a novel lysosome-associated small GTPase, is involved in monocytic differentiation of human acute promyelocytic leukemia cells
Yang M, et al.
Biochemical and Biophysical Research Communications, 318(3), 792-799 (2004)
JNK-interacting protein 3 mediates the retrograde transport of activated c-Jun N-terminal kinase and lysosomes
Drerup CM and Nechiporuk AV
PLoS Genetics, 9(2), e1003303-e1003303 (2013)
Shu-Bai Liu et al.
PloS one, 3(3), e1770-e1770 (2008-03-13)
In vertebrates, non-lens betagamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive. A naturally existing 72-kDa complex of

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