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R7647

Sigma-Aldrich

Random Nonamers

for use as primers in cDNA synthesis, 50 μM in H2O

Synonym(s):
Oligodeoxynucleotides
NACRES:
NA.31

Quality Level

grade

for molecular biology

form

liquid

usage

0.1 mL sufficient for 100 RT-PCR reactions

concentration

50 μM in H2O

shipped in

wet ice

storage temp.

−20°C

General description

Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.

Packaging

100 μL in poly bottle

Application

Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers). Random nonamers may be used as universal primers in first strand cDNA synthesis, cDNA library construction, RT-PCR and other applications.
Suitable for:
  • First strand cDNA synthesis
  • Preparing cDNA libraries (with oligo (dT) primers
  • Prepare labeled DNA probes for hybridizations

Components

Random Nonamers are provided as a 50 uM solution in water.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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