Merck
All Photos(1)

10104159001

Roche

DNase I

grade II, from bovine pancreas

Synonym(s):
Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase

biological source

bovine pancreas

Quality Level

form

lyophilized

packaging

pkg of 100 mg

manufacturer/tradename

Roche

technique(s)

DNA extraction: suitable

optimum pH

~7.0

shipped in

wet ice

storage temp.

2-8°C

General description

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[37] DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.[38]

Application

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
  • The isolation of cells from lung, skin and tumor samples
[29][41][42][43]
  • Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †
  • Unit Definition

    One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

    Preparation Note

    Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
    Working concentration: 1 mg/ml
    Do not vortex while dissolving!
    Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

    Other Notes

    For life science research only. Not for use in diagnostic procedures.

    Pictograms

    Health hazard

    Signal Word

    Danger

    Hazard Statements

    Hazard Classifications

    Resp. Sens. 1 - Skin Sens. 1

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    does not flash

    Flash Point(C)

    does not flash

    Certificate of Analysis

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    Certificate of Origin

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