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R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Synonym(s):

Reference dye for qPCR, ROX

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

form

solution

Quality Level

300

usage

sufficient for ≥600 reactions

concentration

100 ×

technique(s)

PCR: suitable

color

red

solubility

water: soluble

storage temp.

2-8°C

Related Categories

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R4526-5ML:
R4526-5ML-KC:
R4526-EW:
R4526-BULK:
R4526-.3ML-KC:
R4526-.3ML:
R4526-20RXN:
R4526PROC:
R4526-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM0825
SLCL5108
SLCK1389
SLCH7431
SLCH7432

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Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
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Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
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This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
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