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P3988

Sigma-Aldrich

20S Proteasome Fraction from rabbit

≥95% (SDS-PAGE), solution

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About This Item

MDL number:
MFCD01091499
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

rabbit

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

solution

mol wt

700 kDa

UniProt accession no.

A0A5F9CPK4
G1SU71

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

rabbit ... PSMA1(100352563) , PSMB1(100343622)

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

20S proteasome fraction from rabbit has been used as a positive control to determine proteasome activity.

Biochem/physiol Actions

Catalytic core of the 26S proteasome that degrades polyubiquitinated proteins.
Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP dependent process.

Physical form

Solution in 50 mM HEPES, pH 7.6, 150 mM sodium chloride, and 1 mM DTT, pH 7.6.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P3988-25UG:
P3988-BULK:
P3988-VAR:
P3988-25UG-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R L Stein et al.
Biochemistry, 35(13), 3899-3908 (1996-04-02)
In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized
Examination of `lipotoxicity? in skeletal muscle of high-fat fed and ob/ob mice
S.M. Turpin
The Journal of Physiology (2009)
R Hough et al.
The Journal of biological chemistry, 262(17), 8303-8313 (1987-06-15)
We have purified two high molecular weight proteases approximately 400-fold from rabbit reticulocyte lysate. Both enzymes hydrolyze 125I-alpha-casein and 4-methylcoumaryl-7-amide peptides with tyrosine, phenylalanine, or arginine at the P1 position. Both are inhibited by hemin, thiol reagents, chymostatin, and leupeptin.
S M Turpin et al.
The Journal of physiology, 587(Pt 7), 1593-1605 (2009-02-11)
Excess lipid accumulation resulting from an elevated supply of plasma fatty acids is linked to the pathogenesis of the metabolic syndrome and heart disease. The term 'lipotoxicity' was coined to describe how lipid accumulation leads to cellular dysfunction and death

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