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P1253

Sigma-Aldrich

Phosvitin from egg yolk

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About This Item

CAS Number:
9008-96-2
MDL number:
MFCD00131908
UNSPSC Code:
12352202
NACRES:
NA.81

biological source

egg yolk

Quality Level

200

form

powder

storage temp.

−20°C

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General description

Phosvitin from egg yolk is a polyanionic phosphoserine rich protein. This phosphoglycoprotein comprises α-helices and β-sheets structural elements, which are poorly organized due to the presence of phosphoserine. It represents about 11% of yolk proteins and is hydrophilic.

Application

Phosvitin from egg yolk has been used:
  • as a control protein for immobilization on-chip for deep purple protein staining methods for monitoring post-translation modifications
  • as a substrate for casein kinase Iε in in vitro casein kinase assay
  • as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantification of phosvitin from egg extracts
  • for immobilization onto Layer-by-Layer (LbL) for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus.
Phosvitin from egg yolk is a calcium chelator and binds to iron present in the yolk. The metal-binding functionality is exploited in mineral-binding bioactive peptide production. It is a nutraceutical and has potential for phosphopeptides production.

Other Notes

A phosophoprotein containing 8-10% phosphorus. Molar N/P ratio approx. 2.7.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P1253-500MG:
P1253-1G:
P1253-50MG:
P1253-VAR:
P1253-BULK:
P1253-100MG:
P1253-250MG:

Certificates of Analysis (COA)

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K Abdelkebir et al.
Acta biomaterialia, 8(9), 3419-3428 (2012-06-12)
Carboxylated, sulfated and/or phosphorylated surfaces are admitted as potential optimal templates for biomimetic deposition of calcium phosphate (CaP) coatings in view of improving implants' osseointegration. Layer-by-layer films were built up consisting of anionic chondroitin sulfate (ChS), a biological carboxylated and
Xiaowei Zhang et al.
Journal of separation science, 34(22), 3295-3301 (2011-10-15)
An attempt was made to develop a new protocol for preparing phosvitin that could be easy scaled up using polyethylene glycol (PEG6000). Influence of PEG6000 concentration and pH of sample solution on phosvitin isolation was investigated. Phosvitin of high purity
Jun Sun et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 915-916, 33-38 (2013-01-15)
Functional immobilized metal affinity magnetic carboxymethyl chitosan nanoparticles (abbreviated as Fe(3)O(4) (PEG+CM-CTS) @ Fe (III)) were conveniently applied for phosvitin phosphopeptides (PPPs) enrichment for the first time. The morphology of magnetic nanoparticles was observed by transmission electron microscope (TEM). It
Khalil Abdelkebir et al.
Langmuir : the ACS journal of surfaces and colloids, 27(23), 14370-14379 (2011-10-20)
Layer-by-Layer (LbL) coatings are promising tools for the biofunctionalization of biomaterials, as they allow stress-free immobilization of proteins. Here, we explore the possibility to immobilize phosvitin, a highly phosphorylated protein viewed as a model of bone phosphoproteins and, as such
Shaohui Wang et al.
The Journal of biological chemistry, 286(25), 22653-22664 (2011-05-03)
How fish embryos that develop externally survive microbial attacks is poorly understood. Here, we clearly demonstrated that the embryo extract of zebrafish and its early embryo both displayed antimicrobial activity against microbes, including pathogenic Aeromonas hydrophila, and phosvitin (Pv), a
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