ML0015

Aspartate Metabolite Library

• Synonym(s): Aspartic acid Metabolite Library
• UNSPSC Code: 12352209
• NACRES: NA.25

Properties

• description: Aspartate pathway
• Quality Level: 100
• form: solid
• application(s): metabolomics
• storage temp.: −20°C

Description

General description

Aspartic acid (or aspartate) is a non-essential amino acid, which means that it is naturally synthesized by mammals. Aspartate presents many biochemical roles:
In the L-conformation, aspartic acid is a building block in the production of proteins, as well as aiding in many bodily functions, including the urea cycle, gluconeogenesis, and Krebs Cycle, a process that generates adenosine triphosphate (ATP). Aspartic acid also works as a neurotransmitter. The D-Aspartate conformation is linked to neurogenesis and endocrine systems.

Application

The Aspartate Metabolite Library is a kit that contains a selection of 23 metabolite involved in Aspartate metabolism.These may be used for general research, as reagents or as reference compounds in analytical procedures.

Biochem/physiol Actions

Aspartate roles and metabolites:

• Aspartate is synthesized by transamination of oxaloacetate through the actions of Aspartate aminotransferase and pyridoxal 5′- phosphate. Aspartyl-tRNA synthase can then couple the aspartate to aspartyl tRNA for protein synthesis.
• Aspartate carries the reducing equivalents in the mitochondrial Malate-Aspartate shuttle, which uses the ready interconversion of aspartate and oxaloacetate.
• N-acetylaspartate synthase, present in the cytoplasm, converts aspartate to N-acetylaspartate, a brain metabolite that regulates dopamine.
• Asparagine is biosynthesized by Asparagine synthetase from aspartate, glutamine, and ATP. Asparagine is involved in the metabolic control of cell functions in nerve and brain tissue.
• Arginosuccinic acid is synthesized from aspartate, citrulline and ATP through the action of Argininosuccinate synthase, one of the enzymes of the urea cycle. In this metabolic pathway, neurotoxic ammonia, produced by protein catabolism, is converted into urea in the liver.
• Fumaric acid is synthesized from Argininosuccinic acid via an Argininosuccinate lyase, which is an enzyme in the Citric Acid Cycle.
• Inosinic acid, aspartic acid and GTP are interconverted to GDP and AMP by the Adenylosuccinate synthetase isozyme 1. This process is involved in the purine nucleotide cycle which regulates nucleotides levels in various tissues.
• Aspartate transcarbamoylase catalyzes the synthesis of N-carbamoyl-L-aspartate from carbamoyl phosphate and aspartate that are involved in the de novo biosynthesis of pyrimidines.
• Beta alanine is formed by decarboxylation of aspartate by Glutamate decarboxylase 1 in the cytoplasm.
• L-aspartate is converted to D-aspartate through the action of a D-aspartate racemase. D-aspartate contributes to the synthesis and release of glucocorticoids, prolactin, oxytocin, and steroids. D-aspartate plays an important role in the brain activity of mammals.

Components

Contains 10 mg each of Aspartate metabolism metabolite standards packaged individually.

Kit Components Also Available Separately

• A2252 Adenosine 5′-monophosphate monohydrate, from yeast, ≥97%
• A2383 Adenosine 5′-triphosphate disodium salt hydrate, Grade I, ≥99%, from microbial
• A5707 Argininosuccinic acid disodium salt hydrate, ≥80%
• 146064 β-Alanine, 99%
• C4135 Carbamyl phosphate disodium salt, ≥80%
• C7629 L-Citrulline, ≥98% (TLC)
• 219096 D-Aspartic acid, ReagentPlus^®, 99%
F6625 Flavin adenine dinucleotide disodium salt hydrate, ≥95% (HPLC), powder
47910 Fumaric acid, ≥99.0% (T)
G7252 Guanosine 5′-diphosphate tris salt from Saccharomyces cerevisiae, Type VI, ≥92.5%
G9002 Guanosine 5′-triphosphate tris salt, ≥93% (HPLC), powder
I2879 Inosine 5′-monophosphate from Saccharomyces cerevisiae, ≥98%
A5006 L-Arginine, reagent grade, ≥98%
A0884 L-Asparagine, ≥98% (HPLC)
11189 L-Aspartic acid, BioUltra, ≥99.5% (T)
G1251 L-Glutamic acid, ReagentPlus^®, ≥99% (HPLC)
G3126 L-Glutamine
00920 N-Acetyl-L-aspartic acid, ≥99.0% (T)
O4126 Oxaloacetic acid, ≥97% (HPLC)
K1750 α-Ketoglutaric acid, ≥98.5% (NaOH, titration)
P9255 Pyridoxal 5′-phosphate hydrate, ≥98%
P8010 Sodium pyrophosphate tetrabasic, ≥95%
69037 Ureidosuccinic acid, 98.0-102.0% (T)

See All (23)

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H318
• Precautionary Statements: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids