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MBD0052

Sigma-Aldrich

Hemin solution

Ready-made microbial growth medium supplement, 0.5 g/L

Synonym(s):

Chlorohemin solution, ChloroprotoporphyrinIX iron(III) solution, Ferriprotoporphyrin IX chloride Solution

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.21

Quality Level

200

form

liquid

usage

supplement for growth media

concentration

0.5 g/L

shipped in

wet ice

storage temp.

2-8°C

General description

Hemin is a pro-oxidant, hemolysis derived-product. It is essential for the growth of heme-deficient organisms such as trypanosomatids. Hemin readymade solution is used as a culture-medium supplement for fastidious anaerobic microorganisms, shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore forming organisms. It is present as a key ingredient compound in common anaerobic growth media, such as Brucella broth, Thioglycolate broth and Reinforced Clostridial Medium.

Application

Hemin solution may be used as a supplement in brain heart infusion-yeast extract-hemin (BHI-YH) medium to determine cholesterol-to-coprostanol reducing activity.

Features and Benefits

  • Convenient readymade solution to speed up media preparation
  • Sterile solution
  • Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples

Other Notes

  1. For 100 ml of medium preparation, it is recommended to add 1 mL hemin ready-made solution after autoclaving the medium, to achieve a final concentration of 5 microgram/ml. The optimal concentration of the supplement may vary for each strain.
  2. The solution is classified as light sensitive. 2. The solution should not be heated and must be added aseptically to autoclaved media cooled to 50-55°C. 3.
  3. Select strains which show turbid growth (24-48 hr.) with Hemin supplementation: Porphyromonas gingivalis, Bifidobacterium longum, Clostridium perfringens, Bacteroides vulgatus and Fusobacterium nucleatum.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MBD0052-BULK:
MBD0052-VAR:
MBD0052-10ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Manual of clinical microbiology
Murray, P.R., E.J. Baron, M.A.Pfaller, F.C. Tenover, and R.H.Yolken
ASME 2013 Gas Turbine India Conference. American Society of Mechanical Engineers, 2013., 2, 94305-95250 (2006)

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