MAK333

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Citric Acid Test Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• input: culture(s); plasma; tissue; serum; urine
• detection method: colorimetric; fluorometric
• relevant disease(s): cancer
• storage temp.: −20°C

Description

General description

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

Application

The Citrate Assay Kit may be used for:

• Cancer Research
• Drug Discovery
• Metabolic Disorders Research

Features and Benefits

Efficient and Precise: Detect Citrate levels ranging from 4 to 400 μM (colorimetric) and 0.5 to 40 μM (fluorimetric) in a 15 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Principle

In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Storage Class Code: 10 - Combustible liquids