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Safety Information

CS0720

Sigma-Aldrich

Citrate Synthase Assay Kit

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

Synonym(s):

Citrate Synthase Activity Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.28

Quality Level

300

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette)
 kit sufficient for 480 reactions (using 96 multiwell plates)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CS(1431)

General description

The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.

Biochem/physiol Actions

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.

Analysis Note

The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.

Kit Components Only

Product No.
Description
  • Assay buffer for citrate synthase 5x 25 mL
  • Bicine buffer 10 mL
  • CelLytic™ M Cell Lysis Reagent 10 mL
  • DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg
  • Acetyl coenzyme A 25 mg
  • Oxaloacetic acid 1 g
  • Citrate synthase (positive control) .1 mL

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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