MAK290

Trypsin Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

• Synonym(s): Trypsin Enzyme Activity Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• detection method: colorimetric
• relevant disease(s): gastrointestinal diseases
• storage temp.: −20°C

Description

General description

Trypsin (EC 3.4.21.4) is a pancreatic serine protease, which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. Tryptic digestion of the protein of interest results in a highly specific cleavage and a limited number of peptide fragments.

Suitability

Suitable for the detection of Trypsin activity in various samples.

Principle

In this assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ= 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10–100 mU (p-NA unit) trypsin in various samples.

Safety Information

• Pictograms: GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H318,H334
• Precautionary Statements: P261 - P280 - P284 - P305 + P351 + P338 - P501
• Hazard Classifications: Eye Dam. 1 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable