B4750

N_α-Benzoyl-DL-arginine β-naphthylamide hydrochloride

trypsin substrate

• Synonym(s): BANA, N_α-Benzoyl-DL-arginine-2-naphthylamide hydrochloride
• Empirical Formula (Hill Notation): C₂₃H₂₅N₅O₂ · HCl
• CAS Number: 913-04-2
• Molecular Weight: 439.94
• Beilstein: 3823291
• EC Number: 213-015-4
• MDL number: MFCD00038940
• UNSPSC Code: 12352204
• PubChem Substance ID: 24891699
• NACRES: NA.32

Properties

• Assay: ≥98% (TLC)
• form: powder
• solubility: DMF: 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: 2-8°C
• SMILES string: Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc3ccccc3c2
• InChI: 1S/C23H25N5O2.ClH/c24-23(25)26-14-6-11-20(28-21(29)17-8-2-1-3-9-17)22(30)27-19-13-12-16-7-4-5-10-18(16)15-19;/h1-5,7-10,12-13,15,20H,6,11,14H2,(H,27,30)(H,28,29)(H4,24,25,26);1H
• InChI key: BNGRKDJZQIGWQF-UHFFFAOYSA-N

Description

Application

N_α-Benzoyl-DL-arginine β-naphthylamide hydrochloride has been used as a substrate in the BANA test to evaluate the inhibitory activity of stefin. It has also been used as a substrate for the determination of Ki values of inhibitors toward trypsin.

Biochem/physiol Actions

N_α-Benzoyl-DL-arginine β-naphthylamide hydrochloride (BANA) helps to determine the activities of cathepsin B and papain. BANA test is also useful in identifying the number of periodontal sites with periodontal pathogens. Presence of volatile sulfur compounds (VSCs), synthesized by oral bacteria in dental plaque or tongue coating can be colorimetrically confirmed by their ability to hydrolyze N-benzoyl-DL-arginine-2-naphthylamide.

Substrates

A chromogenic substrate for trypsin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves