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MAK190

Sigma-Aldrich

ATP Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Synonym(s):

Adenosine triphosphate Assay Kit

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About This Item

EC Number:
213-579-1
UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric
fluorometric

relevant disease(s)

cancer; neurological disorders

storage temp.

−20°C

General description

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.

Application

ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.

Suitability

Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.

Principle

The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.

Other Notes

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Kit Components Only

Product No.
Description
  • ATP Assay Buffer
  • ATP Probe, in DMSO
  • ATP Converter
  • Developer Mix
  • ATP Standard, 1.0 μmole

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

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Cartagena Act

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JAN Code

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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