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MAK087

Sigma-Aldrich

Acid Phosphatase Activity Fluorometric Assay Kit

sufficient for 500 fluorometric tests

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About This Item

EC Number:
231-834-5
UNSPSC Code:
12352200

usage

sufficient for 500 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)
mouse ... ACP1(11431) , ACP2(11432)
rat ... ACP1(24161) , ACP2(24162)

General description

The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of acid phosphatase in serum and other biological samples.

Principle

The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.

replaced by

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Description
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Storage Class Code

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAK087-1KT:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
7D21K04210
5G12K04210
4M20K04210
3K17K04210
B9J160421V

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Significance of increased phosphatase activity of bone at the site of osteoplastic metastases secondary to carcinoma of the prostate gland.
Gutman E B, et al.
Cancer Research, 28(3), 485-495 (1936)
Kapil D Patel et al.
ACS applied bio materials, 2(11), 5190-5203 (2019-11-18)
Providing appropriate biophysical and biochemical cues to the interface is a facile strategy to enhance the osteogenic ability of metallic implants. Here we exploited this through the incorporation of mesoporous bioactive glass nanoparticles (MBGN) at a high content (1:1 by
Ruibin Kuang et al.
Plant physiology, 151(1), 199-209 (2009-07-28)
Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with
Gaurav Datta et al.
Frontiers in cellular neuroscience, 13, 329-329 (2019-08-06)
Human immunodeficiency virus type 1 (HIV-1) associated neuropathy is the most common neurological complication of HIV-1, with debilitating pain affecting the quality of life. HIV-1 gp120 plays an important role in the pathogenesis of HIV neuropathy via direct neurotoxic effects

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