MAK021

High Sensitivity Glucose-6-Phosphate Assay Kit

sufficient for 100 fluorometric tests

• EC Number: 222-938-1
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• relevant disease(s): cancer; hematological disorder
• storage temp.: −20°C

Description

General description

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high. G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. The Glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for quantifying Glucose-6-Phosphate concentrations in a variety of samples.

Principle

Glucose-6-phosphate concentration is by a enzyme assay, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the G6P present. Typical sensitivities of detection for this kit are between 100-500 pmoles. The Glucose-6-Phosphate Assay Kit is much more sensitive than the Glucose-6-Phosphate Colorimetric Assay Kit (MAK014) and is suitable for samples with low concentrations of glucose-6-phosphate.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup