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Safety Information

H8629

Sigma-Aldrich

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder

Synonym(s):

HK/G6PD

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About This Item

MDL number:
MFCD00131322
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

glucose-6-phosphate dehydrogenase activity

≥75 units/mg protein (biuret)

hexokinase activity

≥65 units/mg protein

purified by

crystallization

storage temp.

−20°C

General description

Research area: Cell signaling

Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

Application

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.

Biochem/physiol Actions

Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.

Packaging

Package sizes are based on hexokinase units.

Components

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

Unit Definition

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H8629-5KU:
H8629-500UN:
H8629-BULK:
H8629-VAR:
H8629-200UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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