H6380
Hexokinase from Saccharomyces cerevisiae
lyophilized powder, ≥350 units/mg protein, Protein ≥10 % by biuret
Synonym(s):
ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
About This Item
Recommended Products
biological source
Saccharomyces cerevisiae
Quality Level
form
lyophilized powder
specific activity
≥350 units/mg protein
mol wt
dimer 110 kDa
composition
Protein, ≥10% biuret
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
white
pH
6
application(s)
life science and biopharma
foreign activity
L-Glutamic dehydrogenase ≤0.05%
Creatine phosphokinase, myokinase, 6-phosphogluconic dehydrogenase and alcohol dehydrogenase ≤0.001%
Glucose-6-phosphate dehydrogenase ≤0.005%
Glutathione reductase ≤0.005%
Phosphoglucose isomerase ≤0.002%
storage temp.
2-8°C
Gene Information
bakers yeast ... HXK1(850614) , HXK2(852639)
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General description
Application
- to investigate its amyloid fibril forming tendency using biophysical methods
- in tracking ATP depletion in peroxisomes and reticulocyte lysates in import assays
- in the synthesis of Mg-ADP-actin from Mg-ATP actin monomers
Biochem/physiol Actions
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Unit Definition
Physical form
Other Notes
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
H6380-VAR:
H6380-1.5KU:
H6380-BULK:
Certificates of Analysis (COA)
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