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F6682

Sigma-Aldrich

Anti-Filamin antibody,Mouse monoclonal

clone PM6/317, purified from hybridoma cell culture

Synonym(s):

Anti-ABP-280, Anti-ABPX, Anti-CSBS, Anti-CVD1, Anti-FGS2, Anti-FLN, Anti-FLN-A, Anti-FLN1, Anti-FMD, Anti-MNS, Anti-NHBP, Anti-OPD, Anti-OPD1, Anti-OPD2, Anti-XLVD, Anti-XMVD

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About This Item

MDL number:
MFCD00162292
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PM6/317, monoclonal

form

buffered aqueous solution

mol wt

antigen ~190 kDa

species reactivity

human, monkey, hamster, bovine, canine

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: suitable
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extract of human Jurkat or HEK-293Tcells

isotype

IgG1

UniProt accession no.

P21333

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FLNA(2316)

General description

Filamin, also known as actin-binding protein, is a large flexible V-shaped actin cross-linking cytoplasmic protein present in vertebrates of both muscle and non-muscle cells. It has actin-binding domains at the ends of each arm. It is a polypeptide with molecular weight of approximately 250 kDa and forms dimers in solution. It has a large flexible actin cross-linking protein present in vertebrates in both the muscle and non-muscle cells. The name filamins refers to its filamentous colocalization with actin stress fibers. It has an actin binding domain and a homodimerization domain. Native form comprises of two subunits with one actin-binding site.
Monoclonal Anti-Filamin (mouse IgG1 isotype) is derived from the hybridoma PM6/317 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes.

Immunogen

human platelet membranes

Application

Monoclonal Anti-Filamin antibody is suitable for indirect immunofluorescence and western blot (chemiluminescent) at a concentration of 0.5-1.0μg/mL using whole extract of human Jurkat or HEK-293Tcells.
Monoclonal Anti-Filamin antibody produced in mouse has been used in:
  • immunoblotting
  • immunofluorescent staining
  • immunoprecipitation

Biochem/physiol Actions

Filamin has been primarily identified from smooth muscle but it has also found in macrophages, neutrophils, platelets and other cell types. It is associated in regulation of the cytoskeletal network by cross-linking actin and linking cell membrane to the cytoskeleton. It is also linked in regulating intracellular signaling pathways, responsible for skeletal development. It binds to the ring zinc finger domain of tumor necrosis factor (TNF) and receptor-associated factor 2 (TRAF2). Filamin cross-links with actin and promotes its aggregation and gelation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F6682-25UL:
F6682-VAR:
F6682-BULK:
F6682-200UL:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell competition is a cell-cell interaction by which a cell compares its fitness to that of neighboring cells. The cell with the relatively lower fitness level is the "loser" and actively eliminated, while the cell with the relatively higher fitness
Structure and Organization of Actin Filaments
Cooper GM et al.
Cell (2000)
K Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(11), 4483-4486 (1975-11-01)
A new high-molecular-weight protein, named filamin, was isolated from chicken gizzard. In chicken gizzard, filamin is present in an amount approximately 30-40% of that of myosin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of highly purified filamin revealed a single polypeptide of
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