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Safety Information

F2006

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

EC Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75

product name

Fibronectin human plasma, lyophilized powder, BioReagent, suitable for cell culture

biological source

human plasma

Quality Level

sterility

sterile

product line

BioReagent

Assay

≥85% (SDS-PAGE)

form

lyophilized powder

mol wt

450 kDa

packaging

pkg of 5 x 5 mg
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

>1 mg/mL protein

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

impurities

HBSAG, none detected
HCV, none detected
HIV-1/HIV-2, none detected

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

UniProt accession no.

Binding Specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous in nature and form functional unit arranged linearly on two subunit arms. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of fibronectin protein due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from human plasma is used for the following applications:
  • Used in cell seeding and neuronal differentiation (as one of the components in expansion medium for the re-suspension of CBSCs (Cord blood stem cells) and is also used in differentiation medium)
  • Used in cell culture (the culture chamber was coated with fibronectin for the expression of HEK293 cells)
  • In-vitro fibronectin (FN) pulldown assays
  • Protein specificity
  • Used in cell migration study

Biochem/physiol Actions

Fibronectin (FN) plays an essential role in multiple biological functions such as, angiogenesis, cell migration cell-to-cell adhesion, cell-to-basement-membrane attachment, clot stabilization, embryogenesis, differentiation, wound healing, as well as binding of pathogen to mammalian cells and extracellular matrix. Studies in Chinese Han population show that mutations in this gene might be linked with susceptibility to knee osteoarthritis (OA). Splice variant extra domain-B (EDB) of FN is a hallmark of tumor angiogenesis, and with increased matrix stiffness there is an increase in the production of this isoform by endothelial cells. In colorectal cancer (CRC), EDA is linked with metastasis, vasculogenesis and tumorigenesis and promotes epithelial-mesenchymal transition.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B, hepatitis C and syphilis. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F2006-1MG-PW:
F2006-2MG-PW:
F2006-5MG-PW:
F2006-BULK-N:
F2006-2MG:
F2006PROC:
F2006-5MG:
F2006-BULK:
F2006-5X5MG:
F2006-PH:
F2006-1MG:
F2006-VAR:


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Francois Bordeleau et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(27), 8314-8319 (2015-06-25)
Alternative splicing of proteins gives rise to different isoforms that play a crucial role in regulating several cellular processes. Notably, splicing profiles are altered in several cancer types, and these profiles are believed to be involved in driving the oncogenic
Chia-Wen Chang et al.
Lab on a chip, 14(19), 3762-3772 (2014-08-07)
This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) hybrid microfluidic device capable of performing cell culture under combinations of chemical and oxygen gradients. The microfluidic device is constructed of two PDMS layers with microfluidic channel patterns separated by a thin PDMS membrane.
Timothy E Quan et al.
Methods in molecular medicine, 135, 423-434 (2007-10-24)
Fibrocytes circulate in the peripheral blood, produce collagen and other matrix proteins, and express cell surface markers indicative of a hematopoetic origin distinguishing them from fibroblasts. Circulating fibrocytes were first identified in 1994 in a model system of wound repair
Kerim B Kaylan et al.
Integrative biology : quantitative biosciences from nano to macro, 8(12), 1221-1231 (2016-11-02)
Carcinoma progression is influenced by interactions between epithelial tumor cells and components of their microenvironment. In particular, cell-extracellular matrix (ECM) interactions are known to drive tumor growth, metastatic potential, and sensitivity or resistance to therapy. Yet the intrinsic complexity of
Marianne J van den Heuvel et al.
Journal of virology, 79(13), 8164-8170 (2005-06-16)
Bovine leukemia virus (BLV) is a deltaretrovirus that infects cattle worldwide. In agriculturally intensive regions, approximately 30% of dairy cows are BLV infected. Like the human T-cell leukemia virus (HTLV), there is a lengthy period of viral quiescence after initial

Articles

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Protocols

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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