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Safety Information

EMS0007

Sigma-Aldrich

Recombinant Trypsin Dimethlyated

Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

Synonym(s):

Dimethylated rTrypsin, Mass Spectrometry Trypsin, Proteomics grade rTrypsin

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.26

recombinant

expressed in Pichia pastoris

Quality Level

200

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥10,000 units/mg protein

suitability

suitable for mass spectrometry

UniProt accession no.

P00761

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0.

This product is prepared from recombinant trypsin, porcine sequence and the lysine residues have been dimethylated to further restrict autolysis. It is naturally devoid of chymotryptic activity. This high quality trypsin is suitable for proteomics use.
Specific activity: >= 10,000 BAEE units per mg protein.

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

EUH071

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

EMS0007PROC:
EMS0007-100UG:4548173367750
EMS0007-BULK:
EMS0007-PH:
EMS0007-4X100UG:
EMS0007-VAR:
EMS0007-100UG-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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