D8764

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

• Synonym(s): DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
• CAS Number: 9025-64-3
• EC Number: 3.1.22.1 (BRENDA, IUBMB)
• EC Number: 232-801-8
• MDL number: MFCD00130919
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine spleen
• type: Type V
• form: essentially salt-free, lyophilized powder
• specific activity: ≥1,000 units/mg protein
• mol wt: 38 kDa
• concentration: ≥50% protein
• technique(s): DNA extraction: suitable; tissue culture: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• foreign activity: RNase ≤0.1%
• storage temp.: −20°C

Description

Application

Deoxyribonuclease II from bovine spleen has been used:

• as a component of digestion mix to isolate immune cells from colon samples
• to digest retinal explants for single-cell suspension preparation
• for isolation of stromal cells from human colon tissue
• in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Unit Definition

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg²⁺] = 0.83 mM

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable